Journal: Developmental Cell

Article Title: A GBF1-Dependent Mechanism for Environmentally Responsive Regulation of ER-Golgi Transport

doi: 10.1016/j.devcel.2019.04.006

Figure Lengend Snippet:

Article Snippet: Brodsky), mouse anti-p230 (BD Biosciences #611280), mouse anti-GM130 (BD Biosciences #610823), mouse anti-calnexin (Abcam #ab31290), mouse anti-PDI (Invitrogen #MA3-018 (RL77)), rabbit anti-Calreticulin (Affinity Bioreagents #PA3-900), sheep anti-P-selectin (R&D Systems #BBA32), mouse anti-CD63 (Abcam #ab59479), rabbit anti-collagen IV (Abcam #ab6586), rabbit anti-collagen I (Abcam #ab34710), rabbit anti-collagen III (Abcam #ab7778), rabbit anti-collagen VI (Abcam #ab6588), rabbit anti-fibronectin (Sigma #F3648), mouse anti-GFP (Roche #11814460001).

Techniques: Recombinant, Luciferase, Software

Journal: Scientific Reports

Article Title: P-Selectin preserves immune tolerance in mice and is reduced in human cutaneous lupus

doi: 10.1038/srep41841

Figure Lengend Snippet: ( a) Relative frequency of peripheral blood leukocyte populations of 1.5–3 month-old WT and P-Sel −/− mice. ( b , d ) Percentage of IL-10 + conventional dendritic cells (cDC), plasmacytoid DC (pDC), monocytes, granulocytes and B cells; and frequency of IL-10 and IL-17 producing CD4 + and CD8 + T lymphocytes, in 1.5-month-old ( b ) and >18-month-old ( d ) WT and P-Sel −/− mice. ( c) Representative dot plots of IL-10 + cDCs and IL-10 and IL-17 producing CD4 + T cells in 1.5-months old WT and P-Sel −/− mice. ( e) Phenotyping of CD4 + and CD8 + splenic T lymphocytes according to the expression of the naïve/memory/effector markers CD62L and CD44 in 1.5–3 months-old (upper panels) and >18 month-old (lower panels) WT and P-Sel −/− mice. ( f) Representative dot plots showing the distribution of 1.5–3-month-old mice splenic populations according to the expression of L-Selectin and CD44. In all cases, n = 4 mice per group. In all cases, n = 4 mice per group. Bars represent the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.005, by Student’s two tailed t test.

Article Snippet: Consecutive tissue sections of sun-exposed skin biopsies underwent immunohistochemistry with the Dako REAL EnVision Detection System Peroxidase/DAB+kit (Dako), using as primary antibodies anti-human P-Selectin (10 μg/ml) (R&D Systems; Minneapolis, MN, USA) and anti-human CD31 (1/50) (Abcam).

Techniques: Expressing, Two Tailed Test

Journal: Scientific Reports

Article Title: P-Selectin preserves immune tolerance in mice and is reduced in human cutaneous lupus

doi: 10.1038/srep41841

Figure Lengend Snippet: ( a) Representative photomicrographs (20×) of anti-CD31 and anti-P-Selectin stained skin biopsies of healthy donors and SLE patients (upper panels). Black arrows point blood vessels. ( b) 200% magnification of representative blood vessels from the original images are represented (lower panels). ( c) Classification and quantification of CD31+ dermal blood vessels according to the expression level of P-Selectin (healthy controls, n = 4; SLE patients, n = 4); bars show the mean ± SD. **p < 0.01 by Student’s two tailed t test.

Article Snippet: Consecutive tissue sections of sun-exposed skin biopsies underwent immunohistochemistry with the Dako REAL EnVision Detection System Peroxidase/DAB+kit (Dako), using as primary antibodies anti-human P-Selectin (10 μg/ml) (R&D Systems; Minneapolis, MN, USA) and anti-human CD31 (1/50) (Abcam).

Techniques: Staining, Expressing, Two Tailed Test

Journal: Oncotarget

Article Title: A pentacyclic triterpene natural product, ursolic acid and its prodrug US597 inhibit targets within cell adhesion pathway and prevent cancer metastasis

doi:

Figure Lengend Snippet: (C) Western blotting analysis of UA/US597 on the down-regulation of integrin α6β1 and its downstream signaling regulator (FAK, Src, paxillin and PTEN) expression in HepG2 cells. Data were obtained from 3 separate experiments and were mean ± SD. (* P < 0.05, ** P < 0.01 in comparison with control).

Article Snippet: PE-labeled mouse anti-human ICAM-1, VCAM-1, integrin α5, α6 and integrin β1 mAbs, FITC-labeled mouse anti-human integrin α4 mAb, APC-labeled mouse anti-human E-selectin mAb, PE/FITC mouse IgG1 kappa isotype control and APC mouse IgG1 kappa isotype control antibody were all purchased from Becton Dickinson (BD) PharmingenTM.

Techniques: Western Blot, Expressing, Comparison, Control

Journal:

Article Title: Selectins and integrins but not platelet-endothelial cell adhesion molecule-1 regulate opioid inhibition of inflammatory pain

doi: 10.1038/sj.bjp.0705837

Figure Lengend Snippet: Leukocyte expression of α4 and β2 integrins, L-selectin and opioid peptides. Blood and subcutaneous tissue from inflamed paws were obtained 6 h after induction of unilateral hind paw inflammation, and processed for flow cytometry. (a–c) Surface expression of adhesion molecules on blood granulocytes. (d–f) Surface expression of adhesion molecules by CD45+ cells in the paw tissue. In (a–f), thick black lines represent anti-α4, anti-β2 and anti-L-selectin mAbs, respectively, whereas gray areas represent respective isotype-matched control mAbs. (g–l) Expression of adhesion molecules and opioid peptides by CD45+ cells in the paw tissue. (g–i) Controls (staining with isotype-matched mAbs) for double-staining experiments shown in (j–l), respectively. (j) Expression of opioid peptides by CD45+ cells. A total of 43% of CD45+ cells express opioids. (k) Coexpression of β2 with opioid peptides. Of all CD45+ cells, 1% express opioids alone, 51% express β2 alone and 44% coexpress β2 with opioids.. Opioid-expressing cells constitute 45% of all CD45+ cells (both upper quadrants), and 98% of these cells coexpress β2. (l) Coexpression of L-selectin with opioid peptides. Of all CD45+ cells, 54% express opioids alone, 2.3% express L-selectin alone and 4.2% coexpress L-selectin with opioids. Opioid-expressing cells constitute 58% of all CD45+ cells (both upper quadrants), and 7% of these cells coexpress L-selectin.

Article Snippet: For double immunofluorescence, the following Abs were used: polyclonal rabbit anti-rat β -endorphin (Peninsula Laboratories, Belmont, CA, U.S.A.), monoclonal mouse-anti-rabbit E-selectin crossreacting with rat E-selectin (generously provided by B. Wolitzky, Hoffman-La Roche, Nutley, NJ, U.S.A.), polyclonal rabbit anti-human P-selectin crossreacting with rat P-selectin (BD Biosciences) and monoclonal mouse anti-rat PECAM-1 (clone TLD-3A12; NatuTec GmbH, Frankfurt/Main, Germany).

Techniques: Expressing, Flow Cytometry, Staining, Double Staining

Journal:

Article Title: Selectins and integrins but not platelet-endothelial cell adhesion molecule-1 regulate opioid inhibition of inflammatory pain

doi: 10.1038/sj.bjp.0705837

Figure Lengend Snippet: Expression of P-selectin (a, b), E-selectin (c, d) and PECAM-1 (e, f) with β-endorphin (a–f) in noninflamed (a, c, e) and inflamed (b, d, f) tissue. Subcutaneous tissue from both hind paws was obtained 6 h after induction of unilateral hind paw inflammation, and processed for double immunofluorescence. Staining of adhesion molecules appears in green, and staining of β-endorphin-containing cells appears in red. Bar=20 μm.

Article Snippet: For double immunofluorescence, the following Abs were used: polyclonal rabbit anti-rat β -endorphin (Peninsula Laboratories, Belmont, CA, U.S.A.), monoclonal mouse-anti-rabbit E-selectin crossreacting with rat E-selectin (generously provided by B. Wolitzky, Hoffman-La Roche, Nutley, NJ, U.S.A.), polyclonal rabbit anti-human P-selectin crossreacting with rat P-selectin (BD Biosciences) and monoclonal mouse anti-rat PECAM-1 (clone TLD-3A12; NatuTec GmbH, Frankfurt/Main, Germany).

Techniques: Expressing, Double Immunofluorescence Staining, Staining

Journal:

Article Title: Selectins and integrins but not platelet-endothelial cell adhesion molecule-1 regulate opioid inhibition of inflammatory pain

doi: 10.1038/sj.bjp.0705837

Figure Lengend Snippet: Quantification of P-selectin-, E-selectin- and PECAM-1-expressing blood vessels in the paw

Article Snippet: For double immunofluorescence, the following Abs were used: polyclonal rabbit anti-rat β -endorphin (Peninsula Laboratories, Belmont, CA, U.S.A.), monoclonal mouse-anti-rabbit E-selectin crossreacting with rat E-selectin (generously provided by B. Wolitzky, Hoffman-La Roche, Nutley, NJ, U.S.A.), polyclonal rabbit anti-human P-selectin crossreacting with rat P-selectin (BD Biosciences) and monoclonal mouse anti-rat PECAM-1 (clone TLD-3A12; NatuTec GmbH, Frankfurt/Main, Germany).

Techniques:

Journal:

Article Title: Selectins and integrins but not platelet-endothelial cell adhesion molecule-1 regulate opioid inhibition of inflammatory pain

doi: 10.1038/sj.bjp.0705837

Figure Lengend Snippet: Effects of selectin and integrin blockade on the number of circulating leukocytes

Article Snippet: For double immunofluorescence, the following Abs were used: polyclonal rabbit anti-rat β -endorphin (Peninsula Laboratories, Belmont, CA, U.S.A.), monoclonal mouse-anti-rabbit E-selectin crossreacting with rat E-selectin (generously provided by B. Wolitzky, Hoffman-La Roche, Nutley, NJ, U.S.A.), polyclonal rabbit anti-human P-selectin crossreacting with rat P-selectin (BD Biosciences) and monoclonal mouse anti-rat PECAM-1 (clone TLD-3A12; NatuTec GmbH, Frankfurt/Main, Germany).

Techniques: